4326595

CD3 Mouse anti-Human, PE, Clone: UCHT1, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD3

Applications

Flow Cytometry

Conjugate

PE

Regulatory Status

RUO

Formulation

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype

IgG1 κ

Purification Method

Affinity Purified

Clone

UCHT1

Classification

Monoclonal

Host Species

Mouse

Target Species

Human

Immunogen

Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Description

  • The UCHT1 monoclonal antibody specifically binds to the human CD3ε-chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex
  • CD3ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes
  • Studies from the HLDA Workshop show that this antibody is mitogenic for CD3ε-positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody
  • CD3 plays a central role in signal transduction during antigen recognition
  • The UCHT1 antibody stains both surface and intracellular CD3ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3ε.

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Antigen:
CD3

Applications:
Flow Cytometry

Conjugate:
PE

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Clone:
UCHT1

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Immunogen:
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Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Antigen:
CD4

Applications:
Flow Cytometry

Conjugate:
FITC

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Clone:
RPA-T4

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Immunogen:
__

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Antigen:
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Applications:
Flow Cytometry

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Regulatory Status:
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Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Clone:
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Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Immunogen:
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Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Antigen:
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Applications:
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Conjugate:
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Regulatory Status:
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Formulation:
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Isotype:
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Purification Method:
__

Clone:
__

Classification:
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Host Species:
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Target Species:
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Immunogen:
__

Primary or Secondary:
__

Content And Storage:
__