CD8 Mouse anti-Human, Brilliant Violet 421, Clone: RPA-T8, BD

Description

• The RPA-T8 monoclonal antibody specifically binds to CD8 alpha (CD8α)
• CD8α is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily
• CD8α is expressed by the majority of thymocytes, by subpopulations of αβ T cells and γδ T cells and by some NK cells
• Cell surface CD8α is expressed either as a disulfide-linked homodimer (CD8αα) or as a heterodimer (CD8αβ) when disulfide-bonded to a CD8 beta chain (CD8β)
• CD8-positive αβ T cells coexpress both CD8αα homodimers and CD8αβ heterodimers whereas some γδ T cells and NK cells express CD8αα homodimers
• CD8 plays important roles in T cell activation and selection
• The extracellular IgSF domain of CD8α binds to a non-polymorphic determinant on HLA class I molecules (α3 domain) and enables CD8 to function as a co-receptor with MHC class I-restricted TCR during T cell recognition of antigen
• The cytoplasmic domain of CD8α associates with Lck, a Src family protein tyrosine kinase that is involved in intracellular signaling
• The RPA-T8 and HIT8a (Cat
• No
• 555337) monoclonal antibodies are not cross-blocking
• The antibody was conjugated to Brilliant Violet 421 and has been developed in collaboration with Sirigen
• With an Ex Max of 407-nm and Em Max at 421-nm, Brilliant Violet 421 can be excited by the violet laser and detected in the standard Pacific Blue filter set (eg, 450/50-nm filter)
• Brilliant Violet 421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• Host Species: Mouse Clone: RPA-T8 Isotype: IgG1, κ Species Reactivity: Human Flow Cytometry