4358231

CD25 Mouse anti-Human, Brilliant Violet 421, Clone: M-A251, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD25

Applications

Flow Cytometry

Conjugate

Brilliant Violet 421

Regulatory Status

RUO

Formulation

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone

M-A251

Classification

Monoclonal

Host Species

Mouse

Target Species

Human

Isotype

IgG1 κ

Purification Method

Affinity Purified

Description

IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55

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Description

  • The M-A251 monoclonal antibody specifically binds to the 55 kDa type I transmembrane glycoprotein known as the low-affinity interleukin-2 receptor alpha chain subunit (IL-2Rα)
  • CD25 is expressed on regulatory T cells and on activated lymphocytes (T and B) and monocytes
  • It associates with the IL-2Rβ/CD122 and the IL-2Rγ/CD132 receptor chains to form the high-affinity IL-2R complex
  • CD25 expression on T and B lymphocytes is upregulated by antigenic or mitogenic stimulation
  • Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses
  • The antibody was conjugated to Brilliant Violet 421 and has been developed in collaboration with Sirigen
  • With an Ex Max of 407-nm and Em Max at 421-nm, Brilliant Violet 421 can be excited by the violet laser and detected in the standard Pacific Blue filter set (eg, 450/50-nm filter)
  • Brilliant Violet 421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
  • Host Species: Mouse Clone: M-A251 Isotype: IgG1, κ Species Reactivity: Human Flow Cytometry

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Show Difference

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Antigen:
CD25

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 421

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
M-A251

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
IL-2R; IL2RA; IL-2Rα; TCGFR; TAC antigen; p55

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4358232

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Antigen:
CD27

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 421

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
M-T271

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
TNFRSF7; TNF receptor superfamily, member 7; T14; Tp55; S152

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Antigen:
CD38

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 421

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
HIT2

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
T10; ADP-ribosyl cyclase 1; Cyclic ADP-ribose hydrolase 1

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Antigen:
CD127

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 421

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
hIL-7R-M21

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
IL-7R; IL7R; IL7RA; IL-7Rα; IL-7R-alpha; Interleukin-7 Receptor alpha