44-550-G
Phospho-IRS1 (Ser616) Polyclonal Antibody, Invitrogen™
Manufacturer: Invitrogen
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each | 44-550-G-Each | In Stock | ₹ 35,306.33 |
44-550-G - Each
In Stock
Quantity
1
Base Price: ₹ 35,306.33
GST (18%): ₹ 6,355.139
Total Price: ₹ 41,661.469
Antigen
Phospho-IRS1 (Ser616)
Classification
Polyclonal
Formulation
Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide, pH 7.3
Gene Accession No.
P35568, P35569, P35570
Gene Symbols
IRS1
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of IRS-1 that contains serine 616. The sequence is conserved in many species including human, mouse, and rat
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Human, Mouse, Rat
Product Type
Antibody
Isotype
IgG
Applications
Immunohistochemistry (Paraffin), Western Blot
Conjugate
Unconjugated
Gene
IRS1
Gene Alias
G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185
Host Species
Rabbit
Purification Method
Antigen affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
16367, 25467, 3667
Content And Storage
-20°C
Form
Liquid
Related Products
Description
- Purified from rabbit serum by sequential epitope-specific chromatography, this product contains enough material for 10 mini-blots
- This antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated insulin receptor substrate-1 (IRS-1)
- The final product is generated by affinity chromatography using IRS-1 derived peptide that is phosphorylated at serine 616
- The antibody has been used in western blotting
- Previous lots of this antibody have been used in immunohistochemistry
- In western analysis the positive control used was human embryonic kidney cell line transfected with a vector encoding IRS-1 (293T) and stimulated with a phorbol ester, TPA
- IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4)
- IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues
- An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo
- Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II
- Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade
- IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.
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Antigen: Phospho-IRS1 (Ser616)
Classification: Polyclonal
Formulation: Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide, pH 7.3
Gene Accession No.: P35568, P35569, P35570
Gene Symbols: IRS1
Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of IRS-1 that contains serine 616. The sequence is conserved in many species including human, mouse, and rat
Quantity: 100 μL
Primary or Secondary: Primary
Target Species: Human, Mouse, Rat
Product Type: Antibody
Isotype: IgG
Applications: Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Gene: IRS1
Gene Alias: G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185
Host Species: Rabbit
Purification Method: Antigen affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 16367, 25467, 3667
Content And Storage: -20°C
Form: Liquid
Antigen:
Phospho-IRS1 (Ser616)
Classification:
Polyclonal
Formulation:
Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide, pH 7.3
Gene Accession No.:
P35568, P35569, P35570
Gene Symbols:
IRS1
Immunogen:
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of IRS-1 that contains serine 616. The sequence is conserved in many species including human, mouse, and rat
Quantity:
100 μL
Primary or Secondary:
Primary
Target Species:
Human, Mouse, Rat
Product Type:
Antibody
Isotype:
IgG
Applications:
Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Gene:
IRS1
Gene Alias:
G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185
Host Species:
Rabbit
Purification Method:
Antigen affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
16367, 25467, 3667
Content And Storage:
-20°C
Form:
Liquid
Antigen: __
Classification: __
Formulation: __
Gene Accession No.: __
Gene Symbols: __
Immunogen: __
Quantity: 1 x 384-well plate
Primary or Secondary: __
Target Species: __
Product Type: Rnase P Instrument Verification Plate
Isotype: __
Applications: __
Conjugate: __
Gene: __
Gene Alias: __
Host Species: __
Purification Method: __
Regulatory Status: __
Gene ID (Entrez): __
Content And Storage: Store Calibration Plates at -15°C to -25°C
Form: __
Antigen:
__
Classification:
__
Formulation:
__
Gene Accession No.:
__
Gene Symbols:
__
Immunogen:
__
Quantity:
1 x 384-well plate
Primary or Secondary:
__
Target Species:
__
Product Type:
Rnase P Instrument Verification Plate
Isotype:
__
Applications:
__
Conjugate:
__
Gene:
__
Gene Alias:
__
Host Species:
__
Purification Method:
__
Regulatory Status:
__
Gene ID (Entrez):
__
Content And Storage:
Store Calibration Plates at -15°C to -25°C
Form:
__
Antigen: Phospho-SHP2 (Tyr542)
Classification: Polyclonal
Formulation: Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3
Gene Accession No.: P35235, Q06124
Gene Symbols: PTPN11
Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human SHP2 that contains tyrosine 542. The sequence is conserved in mouse, rat and chicken.
Quantity: 100 μL
Primary or Secondary: Primary
Target Species: Human, Mouse
Product Type: Antibody
Isotype: IgG
Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate: Unconjugated
Gene: PTPN11
Gene Alias: 2700084A17Rik; AW536184; bptp3; CFC; cSH-PTP2; encodes catalytic domain; HCP; HGNC:9644; JMML; METCDS; MGC14433; Noonan syndrome 1; ns1; null; OTTHUMP00000166108; phosphotyrosyl-protein phosphatase; protein tyrosine phosphatase non-receptor type 11; protein tyrosine phosphatase SH-PTP2; protein tyrosine phosphatase, non-receptor type 11; protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1); protein tyrosine phosphatase, non-receptor type 11 S homeolog; protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; protein-tyrosine phosphatase SYP; PTP1C; PTP1D; PTP-1D; ptp-2; PTP2C; PTP-2C; Ptpn11; ptpn11.S; ptpn11-a; ptpn11-b; SAP-2; SH2 domain-containing protein tyrosine phosphatase-2; Shp2; shp-2; SHPTP2; SH-PTP2; SH-PTP2 protein tyrosine phosphatase non-receptor type 11; SH-PTP2 protein tyrosine phosphatase, non-receptor type 11; SH-PTP3; Syp; Tyrosine-protein phosphatase non-receptor type 11; XELAEV_18010676mg
Host Species: Rabbit
Purification Method: Antigen affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 19247, 5781
Content And Storage: -20°C
Form: Liquid
Antigen:
Phospho-SHP2 (Tyr542)
Classification:
Polyclonal
Formulation:
Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3
Gene Accession No.:
P35235, Q06124
Gene Symbols:
PTPN11
Immunogen:
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human SHP2 that contains tyrosine 542. The sequence is conserved in mouse, rat and chicken.
Quantity:
100 μL
Primary or Secondary:
Primary
Target Species:
Human, Mouse
Product Type:
Antibody
Isotype:
IgG
Applications:
Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
Conjugate:
Unconjugated
Gene:
PTPN11
Gene Alias:
2700084A17Rik; AW536184; bptp3; CFC; cSH-PTP2; encodes catalytic domain; HCP; HGNC:9644; JMML; METCDS; MGC14433; Noonan syndrome 1; ns1; null; OTTHUMP00000166108; phosphotyrosyl-protein phosphatase; protein tyrosine phosphatase non-receptor type 11; protein tyrosine phosphatase SH-PTP2; protein tyrosine phosphatase, non-receptor type 11; protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1); protein tyrosine phosphatase, non-receptor type 11 S homeolog; protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; protein-tyrosine phosphatase SYP; PTP1C; PTP1D; PTP-1D; ptp-2; PTP2C; PTP-2C; Ptpn11; ptpn11.S; ptpn11-a; ptpn11-b; SAP-2; SH2 domain-containing protein tyrosine phosphatase-2; Shp2; shp-2; SHPTP2; SH-PTP2; SH-PTP2 protein tyrosine phosphatase non-receptor type 11; SH-PTP2 protein tyrosine phosphatase, non-receptor type 11; SH-PTP3; Syp; Tyrosine-protein phosphatase non-receptor type 11; XELAEV_18010676mg
Host Species:
Rabbit
Purification Method:
Antigen affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
19247, 5781
Content And Storage:
-20°C
Form:
Liquid
Antigen: __
Classification: __
Formulation: __
Gene Accession No.: __
Gene Symbols: __
Immunogen: __
Quantity: 5 nmol
Primary or Secondary: __
Target Species: __
Product Type: siRNA
Isotype: __
Applications: __
Conjugate: __
Gene: __
Gene Alias: __
Host Species: __
Purification Method: __
Regulatory Status: __
Gene ID (Entrez): __
Content And Storage: The siRNA is provided dried down in a single tube, along with Nuclease-free Water for resuspension, and should be stored at –20°C.
Form: __
Antigen:
__
Classification:
__
Formulation:
__
Gene Accession No.:
__
Gene Symbols:
__
Immunogen:
__
Quantity:
5 nmol
Primary or Secondary:
__
Target Species:
__
Product Type:
siRNA
Isotype:
__
Applications:
__
Conjugate:
__
Gene:
__
Gene Alias:
__
Host Species:
__
Purification Method:
__
Regulatory Status:
__
Gene ID (Entrez):
__
Content And Storage:
The siRNA is provided dried down in a single tube, along with Nuclease-free Water for resuspension, and should be stored at –20°C.
Form:
__