CD169 (Siglec-1) Monoclonal Antibody (SER-4), APC-eFluor™ 780, eBioscience™, Invitrogen™

Description

• Description: The SER-4 monoclonal antibody reacts with mouse CD169, which is also known as Sialoadhesin and Siglec-1
• CD169 is a 185-kDa member of the Siglec family of adhesion and endocytic receptors that recognize sialylated glycans
• This receptor is expressed on a subset of macrophages that are found with high frequency in the spleen, liver, lymph node, and bone marrow
• CD169 can also be detected on monocytes in the bone marrow and periphery
• This receptor plays a role in cell-cell adhesion, as well as internalization of sialylated virus and bacteria
• This endocytic pathway allows CD169+ macrophages to present lipid antigens to iNKT cells, thereby leading to their activation
• Studies have also demonstrated that direct interaction of CD169 with regulatory T cells inhibits their proliferation
• The SER-4 antibody has been reported to have antagonist activity
• Applications Reported: This SER-4 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This SER-4 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
• This may be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions
• Family members include sialoadhesin, CD22 and CD33
• Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.