4747111

CD3 Mouse anti-Human, BUV395, Clone: UCHT1, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD3

Applications

Flow Cytometry

Conjugate

Brilliant Ultraviolet 395

Regulatory Status

RUO

Formulation

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype

IgG1 κ

Purification Method

Affinity Purified

Description

CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon

Clone

UCHT1

Classification

Monoclonal

Host Species

Mouse

Target Species

Human

Immunogen

Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Description

  • The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex
  • CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes
  • Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody
  • CD3 plays a central role in signal transduction during antigen recognition
  • The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε
  • The antibody was conjugated to BD Horizon™ BUV395 which has been exclusively developed by BD Biosciences as an optimal dye for use on a 355nm laser equipped instrument
  • With an Ex Max at 348nm and an Em Max at 395nm, this dye has virtually no spillover into any other detector
  • BD Horizon™ BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.

Compare Similar Items

Show Difference

Img

Fischer Scientific

4747111

Mouse Monoclonal Antibody...


Antigen:
CD3

Applications:
Flow Cytometry

Conjugate:
Brilliant Ultraviolet 395

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon

Clone:
UCHT1

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Immunogen:
Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Img

Fischer Scientific

4747115

Mouse Monoclonal Antibody...


Antigen:
CD4

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 711

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
L3T4; Leu3a; T-cell surface antigen T4/Leu-3 ; W3/25 ; CD4 antigen (p55)

Clone:
SK3

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Immunogen:
Human Peripheral Blood T Cells

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Fischer Scientific

4747116

Mouse Monoclonal Antibody...


Antigen:
CD4

Applications:
Flow Cytometry

Conjugate:
Brilliant Ultraviolet 395

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
L3T4; Leu3a; T-cell surface antigen T4/Leu-3 ; W3/25 ; CD4 antigen (p55)

Clone:
SK3

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Immunogen:
Human Peripheral Blood T Cells

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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4747135

Mouse Monoclonal Antibody...


Antigen:
CD8

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 786

Regulatory Status:
RUO

Formulation:
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32

Clone:
RPA-T8

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Baboon, Cynomolgus, Human, Rhesus Monkey

Immunogen:
Human CD8a

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.