4840433

CD3 Mouse anti-Human, BB515, Clone: UCHT1, BD

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

CD3

Applications

Flow Cytometry

Conjugate

Brilliant Blue 515 (BB515)

Regulatory Status

RUO

Immunogen

Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone

UCHT1

Classification

Monoclonal

Host Species

Mouse

Target Species

Human

Isotype

IgG1 κ

Purification Method

Affinity Purified

Description

CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon

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Description

  • The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex
  • CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes
  • Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody
  • CD3 plays a central role in signal transduction during antigen recognition
  • The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε
  • The antibody was conjugated to BD Horizon™ BB515 which was developed exclusively by BD Biosciences
  • With an excitation max of 490nm and an emission max of 515nm, BD Horizon BB515 can be excited by the 488nm laser and detected in a standard FITC set (e.g
  • 530/30-nm filter)
  • This dye provides a much brighter alternative to FITC with less spillover into the PE detector.

Compare Similar Items

Show Difference

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Fischer Scientific

4840433

Mouse Monoclonal Antibody...


Antigen:
CD3

Applications:
Flow Cytometry

Conjugate:
Brilliant Blue 515 (BB515)

Regulatory Status:
RUO

Immunogen:
Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
UCHT1

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon

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4840447

Mouse Monoclonal Antibody...


Antigen:
CD4

Applications:
Flow Cytometry

Conjugate:
Brilliant Blue 515 (BB515)

Regulatory Status:
RUO

Immunogen:
__

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
RPA-T4

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
L3T4; T-cell surface antigen T4/Leu-3; W3/25; CD4 antigen (p55)

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Antigen:
CD56

Applications:
Flow Cytometry

Conjugate:
Brilliant Blue 515 (BB515)

Regulatory Status:
RUO

Immunogen:
Human NK Cells

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
B159

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Human

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
NCAM1; NCAM-1; Neural cell adhesion molecule 1; NCAM; NKH1; MSK39

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4840472

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Antigen:
CD8

Applications:
Flow Cytometry

Conjugate:
Brilliant Violet 711

Regulatory Status:
RUO

Immunogen:
Human CD8a

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Clone:
RPA-T8

Classification:
Monoclonal

Host Species:
Mouse

Target Species:
Baboon, Cynomolgus, Human, Rhesus Monkey

Isotype:
IgG1 κ

Purification Method:
Affinity Purified

Description:
CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32