KLRG1 Monoclonal Antibody (13F12F2), APC-eFluor™ 780, eBioscience™, Invitrogen™

Description

• Description: This 13F12F2 monoclonal antibody reacts with human killer cell lectin-like receptor subfamily G, member 1 (KLRG1), a type II transmembrane inhibitor receptor of the C-type lectin superfamily that contains an ITIM domain
• This inhibitory receptor is expressed on subsets of gamma-delta T cells, NK (CD56dim), CD8+ and CD4+ T Cells
• KLRG1 is expressed primarily by cells with an effector/memory phenotype that are short-lived, but capable of immediate effector cell function
• Cadherin-E, -N, and -R are ligands for KLRG1
• Cadherin/KLRG1 interaction inhibits cytolytic activity and proliferation
• The percentage of KLRG1 positive cells can vary considerably, depending on antigen experience
• The clones 13F12F2 and 13A2 appear to recognize a similar epitope based on cross-blocking studies
• Applications Reported: This 13F12F2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 13F12F2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm)
• KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains
• These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1
• KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells
• NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.