Rat anti-Mouse IgG1 Secondary Antibody, eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody M1-14D12 recognizes Mouse IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy
• The monoclonal does not recognize other mouse isotypes nor does it crossreact to rat IgG1 or any rat isotype antibodies
• Applications Reported: The 1C10 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This M1-14D12 antibody has been tested by flow cytometric analysis of staining with a mouse IgG1 primary antibody
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 450 is an alternative to Pacific Blue™
• eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target
• Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g
• HRP, AP, fluorescence)
• They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody
• Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e
• immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.