Gata-3 Monoclonal Antibody (TWAJ), eFluor™ 450, eBioscience™, Invitrogen™

Description

• The BMA12 monoclonal antibody reacts with mouse CD100, also known as Sema4D
• Sema4D/CD100 is a 150kDa transmembrane protein expressed constitutively on T cells and is involved in the activation of B cells through its interaction with CD72
• Interaction of these two proteins diminishes the negative signaling normally effected by CD72
• CD100 is already expressed strongly on resting T cells and somewhat weakly on B cells, but activation upregulates expression on both cell types
• Antigen expression has been reported in Spleen, thymus and l The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins
• Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes
• GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain
• GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages
• GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers
• Labastie et al
• (1994) cloned the human gene and the 5-prime end of the mouse gene
• The human gene comprises 6 exons distributed over 17 kb of DNA
• Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.