CD337 (NKp30) Monoclonal Antibody (AF29-4D12), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: This AF29-4D12 monoclonal antibody reacts with human CD337, which is also known as NKp30
• Expressed on NK cells, CD337 is the predominant member of the natural cytotoxicity receptor family that also includes NKp46 and NKp44
• CD337 is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor, virus-infected, and immature dendritic cells
• Several ligands for this receptor have been reported, including B7-H6
• The AF29-4D12 antibody has been reported to crossreact to rhesus macaque
• Applications Reported: This AF29-4D12 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This AF29-4D12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 450 is an alternative to Pacific Blue™
• eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The protein encoded by this gene is a natural cytotoxicity receptor (NCR) that may aid NK cells in the lysis of tumor cells
• The encoded protein interacts with CD3-zeta (CD247), a T-cell receptor
• A single nucleotide polymorphism in the 5' untranslated region of this gene has been associated with mild malaria suceptibility
• Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010].