CD94 Monoclonal Antibody (DX22), PE, eBioscience™, Invitrogen™

Description

• Description: The DX22 monoclonal antibody reacts with human CD94, a 70 kDa type II transmembrane glycoprotein
• CD94 belongs to the C-type lectin superfamily and is present as a heterodimer with NKG2 on the surface
• CD94 is expressed by NK cells, a subset of gd T cells, and NKT cells and plays an important role in adhesion and activation of NK cell lineage
• Applications Reported: The DX22 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This DX22 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Natural killer (NK) cells, like CD94, are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation
• Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function
• KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus
• Three transcript variants encoding two different isoforms have been found for this gene.