CD218a (IL-18Ra) Monoclonal Antibody (H44), FITC, eBioscience™, Invitrogen™

Description

• Description: The H44 antibody reacts with the interleukin-18 receptor alpha chain, also known as IL-1Rrp, an IL-1R family member
• IL-18R alpha is expressed on Th1 cells, NK cell subset, neutrophils and IL-12-stimulated tonsillar B cells
• By itself, IL-18R alpha binds IL-18 with low affinity and when complexed with IL-18R beta chain binds IL-18 with high affinity
• The proinflammatory cytokine IL-18 and its receptor complex play an important role in the innate and autoimmune responses
• Applications Reported: H44 has been reported for use in flow cytometric analysis
• Applications Tested: This H44 antibody has been pre-titrated and tested by flow cytometric analysis on peripheral blood cells and Granulocytes
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• IL18R1 is a cytokine receptor that belongs to the interleukin 1 receptor family
• This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction
• IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells
• The gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q.