Mouse Monoclonal Antibody has been used in 13 publications
Manufacturer: Fischer Scientific
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Androgen R/NR3C4 (p Ser213, p Ser210)
1.0 mg/mL
Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
Unconjugated
Mouse
Breast Cancer, Cancer, Phospho Specific
P10275
367
This antibody was developed against a synthetic peptide corresponding to amino acids 207-221 (GRAREAS*GAPTSSKD) of human androgen receptor, containing the serine 213 phosphorylation site: GenBank Accession No. A39248. Note: S* refers to phosphorylated serine in the peptide sequence.
Primary
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
156C135.2
Western Blot 1 - 4 ug/mL, Immunohistochemistry 1:20 - 1:1000, Immunohistochemistry-Paraffin reported in scientific literature (Lin et al (2007))
Monoclonal
Purified
RUO
Human, Mouse, Rat, Canine, Primate
AIS, androgen receptor, DHTRTFM, Dihydrotestosterone receptorHYSP1, HUMARA, NR3C4KD, Nuclear receptor subfamily 3 group C member 4, SMAX1SBMA, spinal and bulbar muscular atrophy
AR
IgG1 κ
Protein G purified
In IGF-1 stimulated LNCaP cells (passage number 38), a ∼110 kDa band was observed. Please see Lin et al. 2003 for additional details. The serine phosphorylation site recognized by this antibody has been alternatively referred to Ser213 (Lee and Chang, 2003) and Ser210 (Lin et al, 2003). Variations in denotation can arise from how the sequence is counted in various GenBank accession numbers. The site is denoted as Ser213 in GenBank Accession No. A39248, which was used to design the immunogen.