4972821
ROCK1 Antibody (154C1465) - (active/cleaved) - BSA Free, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 5 publications
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
cleaved ROCK1
Concentration
0.5 mg/mL
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Apoptosis, Breast Cancer, Cancer, Signal Transduction, Wnt Signaling Pathway
Gene Accession No.
O60259
Gene ID (Entrez)
6093
Immunogen
This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to amino acids 1114-1126 GNLPESRIEGWLS of human ROCK-1. This sequence is 100% conserved between human, chimpanzee, mouse, rat, dog, and rabbit. This immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1.
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone
154C1465
Dilution
Western Blot 1-2 ug/ml, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 21059388)
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Canine, Primate, Rabbit
Gene Alias
EC 2.7.11, EC 2.7.11.1, MGC131603, MGC43611, p160 ROCK-1, p160ROCK, p160-ROCK, PRO0435, Renal carcinoma antigen NY-REN-35, Rho kinase, rho-associated protein kinase 1, Rho-associated, coiled-coil containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase 1
Gene Symbols
ROCK1
Isotype
IgG
Purification Method
Protein G purified
Test Specificity
This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.
Related Products
Description
- ROCK1 Monoclonal specifically detects ROCK1 in Human, Mouse, Rat, Canine, Primate, Rabbit samples
- It is validated for Western Blot, Immunocytochemistry/Immunofluorescence.
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Antigen: cleaved ROCK1
Concentration: 0.5 mg/mL
Applications: Western Blot, Immunocytochemistry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis, Breast Cancer, Cancer, Signal Transduction, Wnt Signaling Pathway
Gene Accession No.: O60259
Gene ID (Entrez): 6093
Immunogen: This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to amino acids 1114-1126 GNLPESRIEGWLS of human ROCK-1. This sequence is 100% conserved between human, chimpanzee, mouse, rat, dog, and rabbit. This immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone: 154C1465
Dilution: Western Blot 1-2 ug/ml, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 21059388)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Canine, Primate, Rabbit
Gene Alias: EC 2.7.11, EC 2.7.11.1, MGC131603, MGC43611, p160 ROCK-1, p160ROCK, p160-ROCK, PRO0435, Renal carcinoma antigen NY-REN-35, Rho kinase, rho-associated protein kinase 1, Rho-associated, coiled-coil containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase 1
Gene Symbols: ROCK1
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.
Antigen:
cleaved ROCK1
Concentration:
0.5 mg/mL
Applications:
Western Blot, Immunocytochemistry, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis, Breast Cancer, Cancer, Signal Transduction, Wnt Signaling Pathway
Gene Accession No.:
O60259
Gene ID (Entrez):
6093
Immunogen:
This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to amino acids 1114-1126 GNLPESRIEGWLS of human ROCK-1. This sequence is 100% conserved between human, chimpanzee, mouse, rat, dog, and rabbit. This immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone:
154C1465
Dilution:
Western Blot 1-2 ug/ml, Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID 21059388)
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Canine, Primate, Rabbit
Gene Alias:
EC 2.7.11, EC 2.7.11.1, MGC131603, MGC43611, p160 ROCK-1, p160ROCK, p160-ROCK, PRO0435, Renal carcinoma antigen NY-REN-35, Rho kinase, rho-associated protein kinase 1, Rho-associated, coiled-coil containing protein kinase 1, Rho-associated, coiled-coil-containing protein kinase 1
Gene Symbols:
ROCK1
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.
Antigen: c-Myc
Concentration: 1 mg/mL
Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In situ PLA
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Autophagy, Cancer, Cancer Stem Cells, Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, Epigenetics, Epitope Tags, Stem Cell Markers, Transcription Factors and Regulators, Tumor Suppressors
Gene Accession No.: P01106
Gene ID (Entrez): 4609
Immunogen: A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc Antibody (9E10). [UniProt# P01106]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone: 90000000000
Dilution: Western Blot 0.5-2.0 ug/ml, Simple Western 1:200, Flow Cytometry 1:50-1:200. Use reported in scientific literature (PMID 21315712), ELISA 1:100-1:2000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200, Immunoblotting reported in scientific literature (PMID 23750001), Proximity Ligation Assay reported in scientific literature (PMID 33298911), Sandwich ELISA reported in scientific literature (PMID 33122198), Flow (Intracellular), Chromatin Immunoprecipitation (ChIP)
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Bovine, Drosophila
Gene Alias: 9E10 anti-Myc, 9E10 Myc, avian myelocytomatosis viral oncogene homolog, BHLHE39, bHLHe39MRTL, Class E basic helix-loop-helix protein 39, c-Myc, MYC, myc proto-oncogene protein, MYCC, myc-related translation/localization regulatory factor, Proto-oncogene c-Myc, Transcription factor p64, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)
Gene Symbols: MYC
Isotype: IgG1 κ
Purification Method: Protein G purified
Test Specificity: Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
Antigen:
c-Myc
Concentration:
1 mg/mL
Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In situ PLA
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Autophagy, Cancer, Cancer Stem Cells, Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, Epigenetics, Epitope Tags, Stem Cell Markers, Transcription Factors and Regulators, Tumor Suppressors
Gene Accession No.:
P01106
Gene ID (Entrez):
4609
Immunogen:
A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc Antibody (9E10). [UniProt# P01106]
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone:
90000000000
Dilution:
Western Blot 0.5-2.0 ug/ml, Simple Western 1:200, Flow Cytometry 1:50-1:200. Use reported in scientific literature (PMID 21315712), ELISA 1:100-1:2000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200, Immunoblotting reported in scientific literature (PMID 23750001), Proximity Ligation Assay reported in scientific literature (PMID 33298911), Sandwich ELISA reported in scientific literature (PMID 33122198), Flow (Intracellular), Chromatin Immunoprecipitation (ChIP)
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Bovine, Drosophila
Gene Alias:
9E10 anti-Myc, 9E10 Myc, avian myelocytomatosis viral oncogene homolog, BHLHE39, bHLHe39MRTL, Class E basic helix-loop-helix protein 39, c-Myc, MYC, myc proto-oncogene protein, MYCC, myc-related translation/localization regulatory factor, Proto-oncogene c-Myc, Transcription factor p64, v-myc avian myelocytomatosis viral oncogene homolog, v-myc myelocytomatosis viral oncogene homolog (avian)
Gene Symbols:
MYC
Isotype:
IgG1 κ
Purification Method:
Protein G purified
Test Specificity:
Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
Antigen: __
Concentration: __
Applications: __
Conjugate: __
Host Species: __
Research Discipline: __
Gene Accession No.: __
Gene ID (Entrez): __
Immunogen: __
Primary or Secondary: __
Content And Storage: __
Clone: __
Dilution: __
Classification: __
Form: __
Regulatory Status: __
Target Species: __
Gene Alias: __
Gene Symbols: __
Isotype: __
Purification Method: __
Test Specificity: __
Antigen:
__
Concentration:
__
Applications:
__
Conjugate:
__
Host Species:
__
Research Discipline:
__
Gene Accession No.:
__
Gene ID (Entrez):
__
Immunogen:
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Primary or Secondary:
__
Content And Storage:
__
Clone:
__
Dilution:
__
Classification:
__
Form:
__
Regulatory Status:
__
Target Species:
__
Gene Alias:
__
Gene Symbols:
__
Isotype:
__
Purification Method:
__
Test Specificity:
__
Antigen: CD31/PECAM-1
Concentration: 1.0 mg/mL
Applications: Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Angiogenesis, Cellular Markers, Cytoskeleton Markers, Embryonic Stem Cell Markers, Endothelial Cell Markers, Extracellular Matrix, Hematopoietic Stem Cell Markers, Immunology, Myeloid Cell Markers, Myeloid derived Suppressor Cell, Signal Transduction
Gene Accession No.: __
Gene ID (Entrez): 5175
Immunogen: This CD31/PECAM-1 Antibody (TLD-3A12) was developed against activated, Lewis rat derived microglial cells .
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone: TLD-3A12
Dilution: Flow Cytometry 1:10-1:100, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:100
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse, Rat
Gene Alias: adhesion molecule, CD31, CD31 antigen, CD31/EndoCAM, EndoCAM, FLJ34100, FLJ58394, GPIIA′, PECA1, PECAM-1, PECAM-1, CD31/EndoCAM, platelet endothelial cell adhesion molecule, platelet endothelial cell adhesion molecule-1, platelet/endothelial cell adhesion molecule, TLD-3A12 CD31, TLD-3A12 Clone
Gene Symbols: PECAM1
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: Recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.
Antigen:
CD31/PECAM-1
Concentration:
1.0 mg/mL
Applications:
Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Angiogenesis, Cellular Markers, Cytoskeleton Markers, Embryonic Stem Cell Markers, Endothelial Cell Markers, Extracellular Matrix, Hematopoietic Stem Cell Markers, Immunology, Myeloid Cell Markers, Myeloid derived Suppressor Cell, Signal Transduction
Gene Accession No.:
__
Gene ID (Entrez):
5175
Immunogen:
This CD31/PECAM-1 Antibody (TLD-3A12) was developed against activated, Lewis rat derived microglial cells .
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Clone:
TLD-3A12
Dilution:
Flow Cytometry 1:10-1:100, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:100
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse, Rat
Gene Alias:
adhesion molecule, CD31, CD31 antigen, CD31/EndoCAM, EndoCAM, FLJ34100, FLJ58394, GPIIA′, PECA1, PECAM-1, PECAM-1, CD31/EndoCAM, platelet endothelial cell adhesion molecule, platelet endothelial cell adhesion molecule-1, platelet/endothelial cell adhesion molecule, TLD-3A12 CD31, TLD-3A12 Clone
Gene Symbols:
PECAM1
Isotype:
IgG1
Purification Method:
Protein G purified
Test Specificity:
Recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.