Applications:
Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Free Floating), Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In situ PLA, In vitro Assay, KnockDown
Research Discipline:
Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
Gene Accession No.:
Q16665
Immunogen:
This HIF-1 alpha Antibody (H1alpha67) was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Dilution:
Western Blot 1:500, Simple Western, Chromatin Immunoprecipitation 1 - 5 ug/IP. Use reported in scientific literature, Flow Cytometry 1:10 - 1:1000, ELISA 1:100 - 1:2000. Use reported in scientific literature (PMID 20042684), Immunohistochemistry 1:20-1:50, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:20-1:50, Immunohistochemistry-Frozen 1:20-1:50, Immunoassay reported in scientific literature (PMID 26147748), Immunoblotting reported in multiple pieces of scientific literature, In vitro assay reported in multiple pieces of scientific literature, Gel Super Shift Assays 1:1 - 1:100. Use reported in scientific literature (PMID 22411794), Proximity Ligation Assay reported in scientific literature (PMID 27595394), Tissue Culture Substratum, Ligand Activation reported in scientific literature (PMID 26147748), Immunohistochemistry Free-Floating reported in scientific literature (PMID 33242463), Chromatin Immunoprecipitation (ChIP)
Classification:
Monoclonal
Target Species:
Human, Mouse, Rat, Porcine, Bovine, Canine, Feline, Ferret, Monkey, Primate, Rabbit, Sheep, Xenopus species
Gene Alias:
AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, H1alpha67, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1
Purification Method:
Protein G purified
Molecular Weight of Antigen:
93 kDa