beta-NGF Monoclonal Antibody, PeproTech®, Invitrogen™

Description

• AA Sequence of recombinant protein: SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA
• Preparation: Produced in mice immunized with highly pure Recombinant Human beta-NGF
• Anti-Human beta-NGF-specific antibody was purified from cell culture by Protein G affinity chromatography
• Sandwich ELISA: Assuming 100 μL/well, a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 200 pg/mL of Recombinant Human beta-NGF when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human beta-NGF (500-P85BT) as the detection antibody at a concentration of approximately 0.5- 1.0 μg/mL
• Western Blot: To detect Human beta-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 μg/mL
• When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human beta-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions
• Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells
• The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites
• Although both types of receptor bind NGF only the high affinity form is capable of signal transduction
• Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex
• Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species
• NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons
• Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons
• Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.