Vimentin Monoclonal Antibody (V9), FITC, eBioscience™, Invitrogen™

Description

• Description: The V9 monoclonal antibody recognizes human Vimentin, a 57 kDa protein that functions as a structural component of intermediate filaments
• Vimentin is expressed in cells derived from the mesenchyme but also in specific populations such as radial glia and immature glial cells, pancreatic precursor cells
• It is proposed to be a marker of cardiac differentiation
• In neural cells, vimentin expression is gradually replaced by neurofilaments
• Reports have also shown surface expression of vimentin on activated macrophages, platelets, as well as apoptotic T cells and neutrophils
• This antibody also recognizes canine (dog), rat and chicken vimentin but does not recognize mouse vimentin
• Applications Reported: This V9 antibody has been reported for use in immunocytochemistry and immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded tissue sections (IHC-P)
• Applications Tested: This V9 antibody has been tested by immunofluorescent staining of paraformaldehyde fixed and permeabilized cells
• This can be used at less than or equal to 1 μg/mL
• It is recommended that the antibody be titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin
• It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane
• Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development
• During the development process, vimentin is exchanged for new, tissue-specific IFPs
• Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets
• Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event
• This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.