CD26 Monoclonal Antibody (2A6), PE, eBioscience™, Invitrogen™

Description

• Description: The 2A6 monoclonal antibody reacts with human CD26, a 110 kDa type II transmembrane dimer
• CD26 is an ectoenzyme with dipeptidyl peptidase IV activity expressed by T cells and is upregulated upon T cell stimulation
• It is thought that CD26 plays a role in interaction with the extracellular matrix molecules and in T cell activation
• Applications Reported: The 2A6 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 2A6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD26 (dipeptidyl peptidase IV, DPP IV), adenosine deaminase (ADA) binding protein) is a homodimeric atypical serine protease belonging to the prolyl oligopeptidase family
• CD26 is expressed on lymphocyte cells and is upregulated during T-cell activation
• CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia
• CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection
• CD26 identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26
• Further, CD26 is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides
• Alterations in CD26 peptidase activity are characteristic of malignant transformation, and the enzymatic activity increases dramatically with tumor grade and severity
• CD26 is expressed in various blood cell types, but also in cells that are histogenetically related to activated fibroblasts
• Alterations in CD26 density have been reported on circulating monocytes and CD4+ T cells during rheumatoid arthritis and systemic lupus erythematosus.