CD45RB Monoclonal Antibody (C363.16A), PE, eBioscience™, Invitrogen™

Description

• Description: The C363.16A monoclonal antibody reacts with the exon B-dependent isoform of mouse CD45
• This CD45 isoform is expressed by thymocytes, peripheral B cells and a subpopulation of T cells at high density and by dendritic cells and macrophages at low density
• CD45 is a protein tyrosine phosphatase and serves an important role in leukocyte signal transduction
• Applications Reported: The C363.16A antibody has been reported for use in flow cytometric analysis
• Applications Tested: The C363.16A antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD45RB is a receptor-type protein tyrosine phosphatase, CD45 glycoprotein
• CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis
• CD45 isoforms differ in their extracellular domains, but share identical transmembrane and cytoplasmic domains
• CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
• CD45RB is expressed in microglia and inflammatory cells
• In addition to involvement in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is involved in DNA fragmentation during apoptosis
• CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells.