CD96 (TACTILE) Monoclonal Antibody (6A6), PE, eBioscience™, Invitrogen™

Description

• Description: This 6A6 monoclonal antibody reacts with mouse CD96, a cell adhesion receptor also known as TACTILE
• A member of the Ig superfamily, CD96 is a single pass transmembrane protein consisting of three extracellular Ig-like domains and a proline/serine/threonine rich region
• CD96 is expressed on resident monocytes and T cells at all developmental stages (including gammadelta T cells, thymocytes, and NK cells)
• In the mouse, this receptor is not expressed on B cells
• Unlike in humans, mouse CD96 does not exist as alternative splice variants
• Reports indicate that CD96 binds CD155/poliovirus receptor and Nectin-1 to mediate NK cell adhesion to target cells and cytotoxicity
• This monoclonal antibody has been reported to block CD96 interaction with CD155 and Nectin-1
• Applications Reported: This 6A6 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 6A6 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0
• CD96 is a cell adhesion receptor also known as TACTILE
• A member of the Ig superfamily, CD96 is a single pass transmembrane protein consisting of three extracellular Ig-like domains and a proline/serine/threonine rich region
• CD96 is expressed on resident monocytes and T cells at all developmental stages (including gamma-delta T cells, thymocytes, and NK cells)
• In the mouse, this receptor is not expressed on B cells
• Unlike in humans, mouse CD96 does not exist as alternative splice variants
• Reports indicate that CD96 binds CD155/poliovirus receptor and Nectin-1 to mediate NK cell adhesion to target cells and cytotoxicity.