CD137 (4-1BB) Monoclonal Antibody (4B4 (4B4-1)), PE, eBioscience™, Invitrogen™

Description

• Description: This 4B4 (4B4-1) monoclonal antibody reacts with human CD137 (also known as 4-1BB or TNFRSF9), which is an inducible member of the TNFR family of costimulatory molecules expressed on T cells, natural killer cells, dendritic cells, granulocytes, and mast cells
• Involved in recruiting TNFR-associated factors (TRAF) 1 and 2, CD137 signaling plays a role in T cell activation, maintaining the survival of activated and CD8 memory T cells, as well as suppressing myelopoiesis and dendritic cell development
• Stimulation of this receptor has also been shown to promote expansion of CD4+CD25+ T regulatory cells \i{ex vivo\i}
• The ligand for CD137, 4-1BBL, is found on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells
• Applications Reported: This 4B4 (4B4-1) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 4B4 (4B4-1) antibody has been tested by flow cytometric analysis on two-day Con A-stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD137, also known as TNFRSF9 or 4-1BB, is an inducible costimulatory molecule expressed mainly on activated T cells
• Its ligand, known as 4-1BBL, is expressed on activated macrophages, mature B cells, hematopoietic stem cells, and myeloid progenitor cells
• CD137 signaling leads to maintaining the survival of activated T cells and CD8+ memory T cells, and clonal expansion of T cells, but also to suppressing myelopoiesis and dendritic cell development
• Triggered CD137 induces a cytokine release profile regulating peripheral monocyte survival
• Soluble forms of CD137 may provide negative control mechanism for some immune responses.