CD244.2 (2B4) Monoclonal Antibody (eBio244F4), PE, eBioscience™, Invitrogen™

Description

• Description: Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non-MHC dependent cytotoxicity
• Expression is found on natural killer (NK) cells and many NKT cells and CD8+ cells
• In C57BL/6 mice, two splice variants exist that differ in the length of the cytoplasmic domain
• The long form functions in an inhibitory manner through signaling through SHP-2, while the short form functions in an activating manner
• The ligand for CD244 is CD48 which is expressed on all hematopoietic cells
• Recent data suggests that CD244 interactions with CD48, but not CD2 interaction with CD48, is essential for IL-2-driven expansion and activation of murine NK cells
• Reports show CD244 expression can discriminate hematopoietic progenitors
• Hematopoietic stem cells (HSC) are highly purified as CD150+CD244-CD48- cells while non-self-renewing multipotent hematopoietic progenitors (MPP) are CD244+CD150-CD48- and the most restricted progenitors are CD48+CD244+CD150-
• eBio244F4 was generated against cell lines expressing CD244
• It reacts with the C57BL/6 but not Balb/c due to polymorphisms present in the immunoglobulin V domain of the protein
• The epitope recognized by eBio244F4 is different from that of 2B4; therefore these antibodies do not cross-block the binding to the respective epitopes
• Applications Reported: This eBio244F4 antibody has been reported for use in flow cytometric analysis
• This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing
• The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity
• Alternatively spliced transcript variants encoding different isoforms have been found for this gene.