CD247 (CD3 zeta) Monoclonal Antibody (6B10.2), PE, eBioscience™, Invitrogen™

Description

• Description: This 6B10.2 monoclonal antibody reacts with human CD247, which is also known as the invariant zeta chain
• The CD3 complex is composed of three monomeric chains (gamma, delta, and ε) and the invariant zeta chain, which exists as a homodimer or as a heterodimer with the η subunit
• Together with the T cell antigen receptor (TCR), the CD3 gamma, delta, ε, and zeta chains comprise the TCR/CD3 complex
• CD3 zeta is expressed on immature and mature thymocytes, as well as T lymphocytes and NK cells
• Signal transduction during T cell activation is mediated by tyrosine phosphorylation of cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) present on the zeta chain
• The 6B10.2 monoclonal antibody recognizes a transmembrane epitope of the CD3 zeta chain
• Therefore, flow cytometric analysis with this antibody requires intracellular staining
• In addition, this antibody has been reported to recognize the zeta chain as a homodimer but not as a monomer
• Applications Reported: This 6B10.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This 6B10.2 antibody has been tested by intracellular flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat
• 88-8824) and protocol
• CD3z complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex
• T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta
• These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11
• The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules, and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits
• CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation
• The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum
• It is never expressed on B or NK cells
• Decreased percentages of T lymphocytes may be observed in some autoimmune diseases
• Diseases associated with CD3z include Immunodeficiency 25 and T-B+ Severe Combined Immunodeficiency Due To Cd3delta/Cd3epsilon/Cd3zeta.