CD300e (IREM-2) Monoclonal Antibody (UP-H2), PE, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody UP-H2 recognizes human IREM-2 (immune receptor expressed in monocytic derived cells) also known as LMIR6, and unofficially CD300E and CD300LE
• It is a member of the CMRF-35 family
• This 34 kDa Ig-like containing receptor is an activating receptor and through it's charged residues in the cytokplasmic domain interacts with ITAM containing adaptor proteins like DAP12
• Expression is found exclusively on monocytes and myeloid DC's
• Expression may be transient as differentiation of monocytes leads to down regulation of IREM-2 yet expression is found on mature DCs
• It has been also noted that myeloid cell lines such as THP-1 and U937 do not express IREM-2
• In vitro addition of UP-H2 antibody either soluble or crosslinked stimulates monocytes
• Applications Reported: This UP-H2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This UP-H2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD300e is a member of the glycoprotein family of cell surface proteins expressed on myeloid cells
• CD300e interacts with the TYRO protein tyrosine kinase-binding protein and is thought to be involved as an activating receptor
• CD300e is expressed on mature myeloid dendritic cells and monocytes, and is down regulated on immature dendritic cells
• Further, CD300e interacts with DAP-12, which mediates activating signals
• The alloreactive response of naïve T cells has been reported to become enhanced by the activation of CD300e
• Diseases associated with CD300e dysfunction include tungiasis and lice infestation.