CD339 (Jagged 1) Monoclonal Antibody (HMJ1-29), PE, eBioscience™, Invitrogen™

Description

• Description: This HMJ1-29 monoclonal antibody reacts with mouse Jagged-1, one of five type I transmembrane proteins that serves as a Notch receptor ligand
• Upon binding the Notch receptor (e.g
• Notch 1-4), Jagged-1 undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase
• The second cleavage event releases an intracellular fragment whose biological function remains controversial
• Jagged-1 is expressed by thymic lymphoid and stromal cells, as well as macrophages and CD8+CD11c+ dendritic cells in the spleen
• The HMJ1-29 antibody is reported to crossreact to rat
• Applications Reported: This HMJ1-29 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HMJ1-29 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein
• Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch
• Mutations that alter the jagged 1 protein cause Alagille syndrome
• Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.