50-112-2556
OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal Antibody (eBio25-D1.16 (25-D1.16)), Biotin, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-112-2556-Each-of-1 | In Stock | ₹ 13,946.28 |
50-112-2556 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 13,946.28
GST (18%): ₹ 2,510.33
Total Price: ₹ 16,456.61
Antigen
OVA257-264 (SIINFEKL) peptide bound to H-2Kb
Classification
Monoclonal
Concentration
0.5 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Host Species
Mouse
Quantity
50 μg
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry, Immunohistochemistry
Clone
eBio25-D1.16 (25-D1.16)
Conjugate
Biotin
Gene Alias
H-2Kb-SIINFEKL; OVA257-264; OVA257-264 H-2Kb; OVA-Kb
Purification Method
Affinity chromatography
Regulatory Status
RUO
Target Species
Mouse
Product Type
Antibody
Isotype
IgG1 κ
Description
- Description: The 25-D1.16 monoclonal antibody reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb, or H-2Kb bound with an irrelevant peptide
- This antibody has proven to be very useful tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo
- Applications Reported: This eBio25-D1.16 (25-D1.16) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining
- Applications Tested: This eBio25-D1.16 (25-D1.16) antibody has been tested by flow cytometric analysis of SIINFEKL-pulsed C57Bl/6 splenocytes
- This can be used at less than or equal to 0.125 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Cells can be pulsed with the SIINFEKL peptide according to the following protocol: 1
- With cells in flow staining buffer, add SIINFEKL peptide to a final concentration of 30 uM
- 2
- Incubate cells at 37°C for 2 hours
- 3
- Wash cells with flow staining buffer
- 4
- Proceed with cell surface staining as normal
- For additional information see the references listed below
- Filtration: 0.2 μm post-manufacturing filtered
- OVA257-264 (SIINFEKL) peptide bound to H-2Kb Monoclonal antibody specifically detects OVA257-264 (SIINFEKL) peptide bound to H-2Kb in Mouse samples
- It is validated for Flow Cytometry, Immunohistochemistry.
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Antigen: OVA257-264 (SIINFEKL) peptide bound to H-2Kb
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 50 μg
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Applications: Flow Cytometry, Immunohistochemistry
Clone: eBio25-D1.16 (25-D1.16)
Conjugate: Biotin
Gene Alias: H-2Kb-SIINFEKL; OVA257-264; OVA257-264 H-2Kb; OVA-Kb
Purification Method: Affinity chromatography
Regulatory Status: RUO
Target Species: Mouse
Product Type: Antibody
Isotype: IgG1 κ
Antigen:
OVA257-264 (SIINFEKL) peptide bound to H-2Kb
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
50 μg
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Applications:
Flow Cytometry, Immunohistochemistry
Clone:
eBio25-D1.16 (25-D1.16)
Conjugate:
Biotin
Gene Alias:
H-2Kb-SIINFEKL; OVA257-264; OVA257-264 H-2Kb; OVA-Kb
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Target Species:
Mouse
Product Type:
Antibody
Isotype:
IgG1 κ
Antigen: CD178 (Fas Ligand)
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Host Species: Armenian Hamster
Quantity: 100 μg
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Applications: Flow Cytometry
Clone: MFL3
Conjugate: Biotin
Gene Alias: ADAM10-processed FasL form; ALPS1B; APL; Apoptosis (APO 1) antigen ligand 1; apoptosis (APO-1) antigen ligand 1; Apoptosis (APO-1) antigen ligand 1 (Fas antigen ligand); apoptosis antigen ligand; APT1LG1; APTL; CD178; CD178 antigen; CD95 ligand; CD95 ligand protein;Generalized lymphoproliferative disease (Gld); Cd95l; CD95-L; CD95L protein; fas antigen ligand; Fas ligand; Fas ligand (TNF superfamily, member 6); Fasl; Fas-L; FasL ICD; FasL intracellular domain; Faslg; Fas-LG; generalized lymphoproliferative disease; gld; mutant tumor necrosis factor family member 6; Receptor-binding FasL ectodomain; sFasL; Soluble Fas ligand; soluble form; SPA; SPPL2A-processed FasL form; TNFL; Tnfsf6; TNLG1A; Tumor necrosis factor (ligand) superfamily member 6; Tumor necrosis factor (ligand) superfamily member 6 (apoptosis (APO-1) antigen ligand 1) (Fas antigen ligand); Tumor necrosis factor (ligand) superfamily, member 6 (apoptosis (APO-1) antigen ligand 1) (Fas antigen ligand); Tumor necrosis factor
Purification Method: Affinity chromatography
Regulatory Status: RUO
Target Species: Mouse
Product Type: Antibody
Isotype: IgG
Antigen:
CD178 (Fas Ligand)
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Host Species:
Armenian Hamster
Quantity:
100 μg
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Applications:
Flow Cytometry
Clone:
MFL3
Conjugate:
Biotin
Gene Alias:
ADAM10-processed FasL form; ALPS1B; APL; Apoptosis (APO 1) antigen ligand 1; apoptosis (APO-1) antigen ligand 1; Apoptosis (APO-1) antigen ligand 1 (Fas antigen ligand); apoptosis antigen ligand; APT1LG1; APTL; CD178; CD178 antigen; CD95 ligand; CD95 ligand protein;Generalized lymphoproliferative disease (Gld); Cd95l; CD95-L; CD95L protein; fas antigen ligand; Fas ligand; Fas ligand (TNF superfamily, member 6); Fasl; Fas-L; FasL ICD; FasL intracellular domain; Faslg; Fas-LG; generalized lymphoproliferative disease; gld; mutant tumor necrosis factor family member 6; Receptor-binding FasL ectodomain; sFasL; Soluble Fas ligand; soluble form; SPA; SPPL2A-processed FasL form; TNFL; Tnfsf6; TNLG1A; Tumor necrosis factor (ligand) superfamily member 6; Tumor necrosis factor (ligand) superfamily member 6 (apoptosis (APO-1) antigen ligand 1) (Fas antigen ligand); Tumor necrosis factor (ligand) superfamily, member 6 (apoptosis (APO-1) antigen ligand 1) (Fas antigen ligand); Tumor necrosis factor
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Target Species:
Mouse
Product Type:
Antibody
Isotype:
IgG
Antigen: IgD
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Host Species: Rat
Quantity: 100 μg
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Applications: Flow Cytometry
Clone: 11-26c (11-26)
Conjugate: Biotin
Gene Alias: IgD; Igh-5; IGHD; immunoglobulin heavy constant delta
Purification Method: Affinity chromatography
Regulatory Status: RUO
Target Species: Mouse
Product Type: Antibody
Isotype: IgG2a κ
Antigen:
IgD
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Host Species:
Rat
Quantity:
100 μg
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Applications:
Flow Cytometry
Clone:
11-26c (11-26)
Conjugate:
Biotin
Gene Alias:
IgD; Igh-5; IGHD; immunoglobulin heavy constant delta
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Target Species:
Mouse
Product Type:
Antibody
Isotype:
IgG2a κ
Antigen: CD338 (ABCG2)
Classification: Monoclonal
Concentration: 0.5 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Host Species: Mouse
Quantity: 100 μg
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Applications: Flow Cytometry
Clone: 5D3
Conjugate: Biotin
Gene Alias: ABC transporter; ABC15; Abcg2; ABCP; AI428558; ATP binding cassette subfamily G member 2 (Junior blood group); ATP-binding cassette sub-family G member 2; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2; ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group); BCRP; Bcrp1; BMDP; Breast cancer resistance protein; breast cancer resistance protein 1 homolog; Broad substrate specificity ATP-binding cassette transporter ABCG2; CD338; CDw338; EST157481; GOUT1; mitoxantrone resistance protein 1; mitoxantrone resistance-associated protein; MRX; multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2; MXR; MXR1; MXR-1; placenta specific MDR protein; placenta-specific ATP-binding cassette transporter; UAQTL1; Urate exporter
Purification Method: Affinity chromatography
Regulatory Status: RUO
Target Species: Human
Product Type: Antibody
Isotype: IgG2b κ
Antigen:
CD338 (ABCG2)
Classification:
Monoclonal
Concentration:
0.5 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Host Species:
Mouse
Quantity:
100 μg
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Applications:
Flow Cytometry
Clone:
5D3
Conjugate:
Biotin
Gene Alias:
ABC transporter; ABC15; Abcg2; ABCP; AI428558; ATP binding cassette subfamily G member 2 (Junior blood group); ATP-binding cassette sub-family G member 2; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2; ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group); BCRP; Bcrp1; BMDP; Breast cancer resistance protein; breast cancer resistance protein 1 homolog; Broad substrate specificity ATP-binding cassette transporter ABCG2; CD338; CDw338; EST157481; GOUT1; mitoxantrone resistance protein 1; mitoxantrone resistance-associated protein; MRX; multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2; MXR; MXR1; MXR-1; placenta specific MDR protein; placenta-specific ATP-binding cassette transporter; UAQTL1; Urate exporter
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Target Species:
Human
Product Type:
Antibody
Isotype:
IgG2b κ