Estrogen Receptor alpha Monoclonal Antibody (tbykt), eBioscience™, Invitrogen™

Description

• Description: The tbykt monoclonal antibody reacts with the human Estrogen Receptor alpha (ERa) molecule
• Estrogen receptor is a member of the superfamily of nuclear receptors, which are ligand-dependent transcription factors
• The human estrogen receptor becomes activated following the binding of estrogen, causing a dissociation of the chaperone protein (Hsp90) and dimerization with either ERa or ERb
• The alpha and beta receptors share some homology within their DNA- and ligand- binding domains, but signal in different ways and play different roles in gene regulation
• Following activation, Era can directly bind to DNA through EREs (estrogen response elements) or through a more indirect pathway by binding to other transcription factors (Sp1, AP-1, NF-kB, FoxA1 and others)
• Era binding to DNA can either upregulate or repress gene expression
• The alpha receptor has been implicated in the development and progression of breast cancer and its presence is indicative of improved survival and a better response to endocrine therapy (anti-estrogen therapy- tamoxifen)
• Applications Reported: This tbykt antibody has been reported for use western blotting, immunohistochemical staining, immunocytochemistry, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections
• Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors
• The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA
• When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins
• There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively)
• Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa))
• Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function
• They also play a role in other tissues such as bone
• Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.