Vinculin Monoclonal Antibody (7F9), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin
• Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions
• Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression
• Vinculin binds to talin, paxillin, and alpha-actinin
• Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis
• Applications Reported: This 7F9 antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections and formalin-fixed paraffin embedded tissue sections, immunocytochemistry, and microscopy
• Applications Tested: This 7F9 antibody has been tested by immunocytochemistry on methanol-fixed or formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 5 μg/mL
• This antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval buffer and can be used at less than or equal to 5 μg/mL
• The antibody has been tested by western blot on reduced and non-reduced cell lysates and can be used at less than or equal to 5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions
• It functions as one of several interacting proteins involved in anchoring F-actin to the membrane
• It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques
• In the assembly of adhesion plaques, the beta subunit of integrin binds to talin
• Talin binds to vinculin that interacts with alpha-actinin and possibly with itself
• Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system
• Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.