CD63 Monoclonal Antibody (NVG-2), APC, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody NVG-2 reacts with mouse CD63, also known as Lysosomal-Associated Membrane Protein 3 (LAMP-3) or tetraspanin 30 (TSPN30), a member of tetraspanin family of proteins characterized by four transmembrane domains
• CD63 is expressed on a variety of cell types of hematopoietic lineage, e.g., granulocytes, B lymphocytes, platelets, as well as cells of non-hematopoietic origin
• It can be found on the cell membrane, late endocytic vesicles, lysosomes, exosomes, and other specialized granules
• On the cell surface, CD63 has been shown to interact with various proteins forming tetraspanin-enriched microdomains (TEM)
• Its high expression on the cell membrane may be indicative of cell activation, hence, CD63 is often used as an activation marker for basophils, platelets and other cells
• Applications Reported: This NVG-2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This NVG-2 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse resident peritoneal exudate cells using the Intracellular Fixation & Permeabilization Buffer Set (cat
• 88-8824) and protocol
• Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
• This can be used at less than or equal to 0.25 μg per test
• CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types
• CD63 is also present in the plasma membrane, usually following cell activation
• Hence, CD63 has become a widely used basophil activation marker
• In mast cells, however, CD63 exposition does not need their activation
• CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels
• CD63 also serves as a T-cell costimulation molecule
• Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas
• CD63 is expressed on activated platelets, and is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation
• CD63 is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells
• CD63 is identical to the melanoma-associated antigen which is ME491 and to the platelet antigen PTLGP40
• Diseases associated with CD63 dysfunction include melanoma and Hermansky-Pudlak Syndrome.