CD68 Monoclonal Antibody (FA-11), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: This FA-11 monoclonal antibody reacts with mouse CD68, known also as macrosialin
• CD68 is a heavily O- and N- glycosylated type I integral membrane protein belonging to the sialomucin family and is closely related to the Lysosomal/endosomal-Associated Membrane Glycoproteins (LAMP)
• Although commonly used as a pan-macrophage marker, low levels of expression have also been observed in Langerhans cells and conventional dendritic cells
• CD68 is mostly localized in the late endosomes and lysosomes with a smaller fraction circulating to the cell surface
• In the steady state, only about 10-15% of CD68 is present at the surface
• Cell stimulation increases expression of CD68, its transport to cell surface, and results in glycoform remodeling
• CD68 binds oxidized LDL and is involved in the process of phagocytosis
• The FA-11 antibody is not recommended for use in immunohistochemistry of formalin-fixed paraffin embedded tissues
• Applications Reported: This FA-11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This FA-11 antibody has been tested by intracellular staining and flow cytometric analysis of mouse bone marrow-derived macrophages using the Intracellular Fixation & Permeabilization Buffer Set (cat
• 88-8824) and protocol
• Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
• CD68 Monoclonal antibody specifically detects CD68 in Mouse samples
• It is validated for Flow Cytometry.