CD68 Monoclonal Antibody (FA-11), eBioscience™, Invitrogen™

Description

• Description: This FA-11 monoclonal antibody reacts with mouse CD68, known also as macrosialin
• CD68 is a heavily O- and N- glycosylated type I integral membrane protein belonging to the sialomucin family and is closely related to the Lysosomal/endosomal-Associated Membrane Glycoproteins (LAMP)
• Although commonly used as a pan-macrophage marker, low levels of expression have also been observed in Langerhans cells and conventional dendritic cells
• CD68 is mostly localized in the late endosomes and lysosomes with a smaller fraction circulating to the cell surface
• In the steady state, only about 10-15% of CD68 is present at the surface
• Cell stimulation increases expression of CD68, its transport to cell surface, and results in glycoform remodeling
• CD68 binds oxidized LDL and is involved in the process of phagocytosis
• The FA-11 antibody is not recommended for use in immunohistochemistry of formalin-fixed paraffin embedded tissues
• Applications Reported: This FA-11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, and immunohistochemical staining of frozen tissue sections
• (Fluorochrome conjugated FA-11 is recommended for use in intracellular flow cytometry.)
• Applications Tested: Anti-Mouse CD68 Purified has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 5 μg/mL
• CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes
• Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions
• CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma
• Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.