50-112-4216
TNF alpha Monoclonal Antibody (MP6-XT22), eFluor™ 450, eBioscience™, Invitrogen™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-112-4216-Each-of-1 | In Stock | ₹ 27,721.44 |
50-112-4216 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 27,721.44
GST (18%): ₹ 4,989.859
Total Price: ₹ 32,711.299
Antigen
TNF alpha
Classification
Monoclonal
Concentration
0.2 mg/mL
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.
P06804
Gene Symbols
Tnf
Purification Method
Affinity chromatography
Regulatory Status
RUO
Gene ID (Entrez)
21926
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Form
Liquid
Applications
Flow Cytometry
Clone
MP6-XT22
Conjugate
eFluor 450
Gene
Tnf
Gene Alias
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Host Species
Rat
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Mouse
Product Type
Antibody
Isotype
IgG1 κ
Related Products
Description
- Description: The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF alpha), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4(+) T cells
- TNF alpha has cytolytic activity against a range of tumor cells and is important in immune regulation
- TNF alpha forms dimers and trimers and also exists as a 26 kDa membrane-bound form
- Applications Reported: This MP6-XT22 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This MP6-XT22 antibody has been tested intracellular staining and flow cytometric analysis of restimulated mouse splenocytes
- This can be used at less than or equal to 0.25 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- eFluor™ 450 is an alternative to Pacific Blue™
- eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
- Please make sure that your instrument is capable of detecting this fluorochome
- Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser
- Filtration: 0.2 μm post-manufacturing filtered
- TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily
- This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR
- TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation
- TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions
- Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice
- Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors
- TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity
- This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
Compare Similar Items
Show Difference
Antigen: TNF alpha
Classification: Monoclonal
Concentration: 0.2 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: P06804
Gene Symbols: Tnf
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 21926
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Applications: Flow Cytometry
Clone: MP6-XT22
Conjugate: eFluor 450
Gene: Tnf
Gene Alias: APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Host Species: Rat
Quantity: 100 μg
Primary or Secondary: Primary
Target Species: Mouse
Product Type: Antibody
Isotype: IgG1 κ
Antigen:
TNF alpha
Classification:
Monoclonal
Concentration:
0.2 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
P06804
Gene Symbols:
Tnf
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
21926
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Applications:
Flow Cytometry
Clone:
MP6-XT22
Conjugate:
eFluor 450
Gene:
Tnf
Gene Alias:
APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor
Host Species:
Rat
Quantity:
100 μg
Primary or Secondary:
Primary
Target Species:
Mouse
Product Type:
Antibody
Isotype:
IgG1 κ
Antigen: Granzyme B
Classification: Monoclonal
Concentration: 0.2 mg/mL
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.: P04187
Gene Symbols: GZMB
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 14939
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Applications: Flow Cytometry
Clone: NGZB
Conjugate: eFluor 450
Gene: GZMB
Gene Alias: AI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E
Host Species: Rat
Quantity: 25 μg
Primary or Secondary: Primary
Target Species: Mouse
Product Type: Antibody
Isotype: IgG2a κ
Antigen:
Granzyme B
Classification:
Monoclonal
Concentration:
0.2 mg/mL
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Accession No.:
P04187
Gene Symbols:
GZMB
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
14939
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Applications:
Flow Cytometry
Clone:
NGZB
Conjugate:
eFluor 450
Gene:
GZMB
Gene Alias:
AI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E
Host Species:
Rat
Quantity:
25 μg
Primary or Secondary:
Primary
Target Species:
Mouse
Product Type:
Antibody
Isotype:
IgG2a κ
Antigen: Phospho-S6 (Ser235, Ser236)
Classification: Monoclonal
Concentration: 5 μL/Test
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.: P62753, P62754
Gene Symbols: RPS6
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 20104, 6194
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Applications: Flow Cytometry
Clone: cupk43k
Conjugate: eFluor 450
Gene: RPS6
Gene Alias: (Rp)S6; 40S ribosomal protein S6; air[8]; air8; anon-WO02059370.61; CG10944; CG10944-PA; CG10944-PB; Dmel\CG10944; Dmel_CG10944; DS6; hemocytes enlarged; hen; l(1)air[8]; l(1)air8; l(1)hen; LD31286p; lethal(1)aberrant immune response[8]; lethal-(1)-haemocytes enlarged; lethal-aberrant-immune-response-8; M(1)7BC; M(1)7C; minute; minute (1) 7BC; OK/SW-cl.2; OTTHUMP00000021121; OTTHUMP00000021122; Phosphoprotein NP33; pp30; ribosomal protein S6; Rp S6; RP11-513M16.6; RPS6; RpS6-PA; RpS6-PB; RS6; S6; S6 ribosomal protein; S6R; Small ribosomal subunit protein eS6; wu:fa92e06; wu:fb64g06; zgc:92237
Host Species: Mouse
Quantity: 100 Tests
Primary or Secondary: Primary
Target Species: Human, Mouse
Product Type: Antibody
Isotype: IgG1 κ
Antigen:
Phospho-S6 (Ser235, Ser236)
Classification:
Monoclonal
Concentration:
5 μL/Test
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.:
P62753, P62754
Gene Symbols:
RPS6
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
20104, 6194
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Applications:
Flow Cytometry
Clone:
cupk43k
Conjugate:
eFluor 450
Gene:
RPS6
Gene Alias:
(Rp)S6; 40S ribosomal protein S6; air[8]; air8; anon-WO02059370.61; CG10944; CG10944-PA; CG10944-PB; Dmel\CG10944; Dmel_CG10944; DS6; hemocytes enlarged; hen; l(1)air[8]; l(1)air8; l(1)hen; LD31286p; lethal(1)aberrant immune response[8]; lethal-(1)-haemocytes enlarged; lethal-aberrant-immune-response-8; M(1)7BC; M(1)7C; minute; minute (1) 7BC; OK/SW-cl.2; OTTHUMP00000021121; OTTHUMP00000021122; Phosphoprotein NP33; pp30; ribosomal protein S6; Rp S6; RP11-513M16.6; RPS6; RpS6-PA; RpS6-PB; RS6; S6; S6 ribosomal protein; S6R; Small ribosomal subunit protein eS6; wu:fa92e06; wu:fb64g06; zgc:92237
Host Species:
Mouse
Quantity:
100 Tests
Primary or Secondary:
Primary
Target Species:
Human, Mouse
Product Type:
Antibody
Isotype:
IgG1 κ
Antigen: Phospho-mTOR (Ser2448)
Classification: Monoclonal
Concentration: 5 μL/Test
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.: P42345, Q9JLN9
Gene Symbols: MTOR
Purification Method: Affinity chromatography
Regulatory Status: RUO
Gene ID (Entrez): 2475, 56717
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
Applications: Flow Cytometry
Clone: MRRBY
Conjugate: eFluor 450
Gene: MTOR
Gene Alias: 2610315D21Rik; AI327068; angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP-rapamycin associated protein (FRAP); FKBP-rapamycin-associated protein FRAP; flat; FRAP; FRAP1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mechanistic target of rapamycin kinase; Mtor; m-TOR; mTORC1; Raft1; Rapamycin and FKBP12 target 1; rapamycin and FKBP12 target-1 protein; rapamycin associated protein FRAP2; rapamycin target protein 1; RAPT1; serine/threonine-protein kinase mTOR; SKS
Host Species: Mouse
Quantity: 100 Tests
Primary or Secondary: Primary
Target Species: Human, Mouse
Product Type: Antibody
Isotype: IgG2a κ
Antigen:
Phospho-mTOR (Ser2448)
Classification:
Monoclonal
Concentration:
5 μL/Test
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Accession No.:
P42345, Q9JLN9
Gene Symbols:
MTOR
Purification Method:
Affinity chromatography
Regulatory Status:
RUO
Gene ID (Entrez):
2475, 56717
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
Applications:
Flow Cytometry
Clone:
MRRBY
Conjugate:
eFluor 450
Gene:
MTOR
Gene Alias:
2610315D21Rik; AI327068; angiopoietin-like factor CDT6; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FKBP12-rapamycin complex-associated protein 1; FKBP-rapamycin associated protein; FKBP-rapamycin associated protein (FRAP); FKBP-rapamycin-associated protein FRAP; flat; FRAP; FRAP1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); mechanistic target of rapamycin kinase; Mtor; m-TOR; mTORC1; Raft1; Rapamycin and FKBP12 target 1; rapamycin and FKBP12 target-1 protein; rapamycin associated protein FRAP2; rapamycin target protein 1; RAPT1; serine/threonine-protein kinase mTOR; SKS
Host Species:
Mouse
Quantity:
100 Tests
Primary or Secondary:
Primary
Target Species:
Human, Mouse
Product Type:
Antibody
Isotype:
IgG2a κ