CD184 (CXCR4) Monoclonal Antibody (2B11), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The 2B11 monoclonal antibody reacts with mouse and human CXCR4, also known as CD184, Fusin, LESTR, or HUMSTR
• Fusin is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells
• In addition it has been shown to play a role in lymphocyte migration
• The ligand for CXCR4 is SDF1
• CXCR4 is required for maintece of granulocyte progenitors in the bone marrow and essential for B cell development
• Expression of CXCR4 is different between mice and human
• In the thymus CXCR4 is restricted to CD4+CD8+ cells while in the spleen predomit expression is found on B lymphocytes
• The monoclonal antibody 2B11 recognizes the amino terminus of CXCR4 and has been reported to have functional activity; it does not block SDF1a but causes internalization of the receptor
• Applications Reported: This 2B11 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 2B11 antibody has been tested by flow cytometric analysis of mouse thymocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells
• CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus
• In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells
• CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation
• CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels
• Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells
• In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development
• CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes
• Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells
• The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.