CD8a Monoclonal Antibody (AMC908), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: This AMC908 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha)
• A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a
• CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells
• Applications Reported: This AMC908 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy
• Applications Tested: This AMC908 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using high pH antigen retrieval and can be used at less than or equal to 5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation
• CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules
• CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I
• CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha
• Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation
• Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells
• Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.