HELIOS Monoclonal Antibody (22F6), PE-eFluor™ 610, eBioscience™, Invitrogen™

Description

• Description: This 22F6 monoclonal antibody reacts with human, mouse and canine Helios
• Helios is a zinc-finger transcription factor first identified for its similarity to Ikaros
• It is expressed in hematopoietic stem cells in both the embryo and adult mice
• While the highest expression is found in regulatory T cells, Helios is not expressed in mature B cells, dendritic cells or myeloid cells
• Helios can form a homodimer as well as heterodimerize with other Ikaros family members to regulate transcription
• Eight isoforms of Helios exist, some of which possess a domit-negative function that has also been found to be upregulated in lymphoid maligcies, including T-acute lymphoblastic leukemia
• 22F6 has been reported to cross react with rat Helios
• Applications Reported: This 22F6 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This 22F6 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse lymph node cells using the using the Foxp3/Transcription Factor Buffer Set (cat
• 00-5523) and protocol
• Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins)
• This can be used at 5 μL (0.03 μg) per test
• This gene encodes a member of the Ikaros family of zinc-finger proteins
• Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development
• This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development
• Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.