GARP Monoclonal Antibody (G14D9), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: The G14D9 monoclonal antibody reacts with human Glycoprotein A Repetitions Predomit (GARP, also known as LRRC32 or Garpin)
• GARP is an approximately 80 kDa glycoprotein that is expressed on the cell surface
• Using northern blot, RT-PCR or microarray analyses, the expression of GARP has been reported in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas
• Protein expression has been observed on megakaryocytes, platelets and activated regulatory T (Treg) cells
• The expression of GARP on the surface of activated Treg cells has been reported to be necessary for their suppressive function, possibly related to its role as a cell surface receptor for LAP/TGF beta
• Applications Reported: This G14D9 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This G14D9 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• GARP, also known as leucine rich repeat containing 32, is a type I membrane protein with twenty-two leucine-rich repeats and a short intracytoplasmic region
• GARP was identified based on the analysis of gene expression profiling of Treg and Th cells following TCR stimulation
• Since GARP is expressed in CD4+, CD25+, FoxP3+ cells (Tregs), it may be a candidate for cell surface marker for Treg cells.