CD4 Monoclonal Antibody (YKIX302.9), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: The YKIX302.9 monoclonal antibody reacts with canine CD4, a cell surface receptor expressed on a majority of thymocytes, a subpopulation of mature T cells and dendritic cells
• In dogs, CD4 is also highly expressed on neutrophils
• CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role in T cell development and homeostasis
• This YKIX302.9 monoclonal antibody has been reported to block MHC II-restricted T cell responses in vivo and in vitro
• Applications Reported: This YKIX302.9 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This YKIX302.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells
• This can be used at 5 µL (0.06 µg) per test
• A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Light sensitivity: This tandem dye is sensitive photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
• 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
• Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV
• CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells
• At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues
• CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck
• It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system
• Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].