CD279 (PD-1) Monoclonal Antibody (RMP1-30), eFluor™ 450, eBioscience™, Invitrogen™

Description

• Description: The RMP1-30 antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily
• PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice
• PD-1 is expressed mainly on activated T and B lymphocytes
• Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC)
• Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells
• RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants
• Applications Reported: This RMP1-30 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This RMP1-30 antibody has been tested by flow cytometric analysis of activated mouse splenocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• eFluor™ 450 is an alternative to Pacific Blue™
• eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm)
• Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC)
• T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC
• PD-1 can bind two different but related ligands, PDL-1 and PDL-2
• Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of &quotedanger signals&quote such as LPS or other molecules associated with bacteria or other pathogens
• Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases
• Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.