CD258 (LIGHT) Monoclonal Antibody (7-3 (7)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: This 7-3 (7) monoclonal antibody reacts with human CD258 (also known as LIGHT), a 29-kDa type II transmembrane protein that is a member of the TNF ligand superfamily related to lymphotoxin-β (LT-β)
• Expressed on activated T cells, particularly CD8+ T cells, LIGHT plays a role in cell survival, inflammation, and tumor eradication
• Furthermore, upon binding its ligand, herpesvirus entry mediator (HVEM), LIGHT induces a costimulatory effect on T cells, enhancing T cell proliferation and cytokine production
• LIGHT has also been shown to indirectly interact with the LT-β receptor
• Applications Reported: This 7-3 (7) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 7-3 (7) antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
• We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
• Please make sure that your instrument is capable of detecting this fluorochrome
• Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms
• Apoptosis is induced by certain cytokines including TNF (tumor necrosis factor) and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 (tumor necrosis factor receptor 1) and Fas
• Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE (interleukin-1-beta converting enzyme)/CED-3 (C
• elegans death receptor-3) protease family
• A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (death associated protein 6)
• Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway
• Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways.