TL1A Monoclonal Antibody (Tandys1a), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: The Tandys1a monoclonal antibody reacts with human and mouse TL1A (TNFSF15)
• TL1A is a member of the TNF ligand family and is expressed by endothelial cells, tissue macrophages, monocyte-derived dendritic cells, plasma cells and lamina propria-derived lymphocytes
• Among peripheral blood cells, TL1A has been shown to be expressed predomitly by CD4+CCR9+ lymphocytes
• Its expression has been shown to be induced by TNF alpha, IL-1 alpha and IFN gamma
• TL1A binds DR3, which has been shown to be specifically upregulated in Th17 cells among CD4+ T cells
• TL1A-deficient dendritic cells are weak in their ability to support Th17 cells in vitro and show decreased severity of EAE
• In vivo, TL1A has been shown to be upregulated in tissue lymphocytes of Crohn's Disease and ulcerative colitis patients
• Applications Reported: This Tandys1a antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis
• Applications Tested: This Tandys1a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells or mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family
• This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells
• The expression of this protein is inducible by TNF and IL-1 alpha
• This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6
• It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells
• This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor
• An additional isoform encoded by an alternatively spliced transcript variant has been reported but the sequence of this transcript has not been determined.