CD137 Ligand (4-1BB Ligand) Monoclonal Antibody (TKS-1), Biotin, eBioscience™, Invitrogen™

Description

• Description: The TKS-1 monoclonal antibody reacts with mouse 4-1BB Ligand (4-1BBL), a type II transmembrane protein and a member of the tumor necrosis factor superfamily
• 4-1BBL is expressed by activated antigen presenting cells including B cells and macrophages
• Interaction of 4-1BBL with 4-1BB (CDw137) is reported to deliver a costimulatory signal important in the T-APC interaction
• Applications Reported: The TKS-1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: The TKS-1 antibody has been tested by flow cytometric analysis of resting and 2-3 day (IgM & CD40)-activated mouse splenocytes
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filtration: 0.2 μm post-manufacturing filtered
• CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily
• CD137L is a co-stimulatory molecule that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation
• Its receptor, CD137/4-1BB, is found on a variety of cells, including inflamed endothelial cells, where its expression enhances extravasation of CD137L expressing monocytes
• Activated T cells also express CD137, and engagement with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity
• CD137L is also expressed on a majority of B-cell lymphomas, including mantle cell lymphoma, follicular lymphoma, and diffuse large B-cell lymphoma (but it is lacking on Hodgkin lymphoma and T-cell lymphoma)
• CD137L is also expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interactions.