CD254 (RANK Ligand) Monoclonal Antibody (IK22/5), Functional Grade, eBioscience™, Invitrogen™

Description

• Description The IK22/5 MAb reacts with mouse CD254 also known as TRANCE (TNF-related activation-induced cytokine receptor) and RANKL (receptor activator of NF-kappa B ligand) and OPGL (Osteoprotegerin Ligand)
• TRANCE is a type II membrane protein with predicted molecular weight of 35 kDa
• Its extracellular domain is most closely related to TRAIL, FasL and TNF
• TRANCE mRNA is upregulated by TCR stimulation and is expressed abundantly by mouse T cells not B cells in mouse thymus and lymph nodes
• TRANCE-deficient mouse lacks osteoclasts
• In addition, TRANCE has been shown to be involved in the interactions between T-dendritic and T-B cells
• It was also found to be critical in osteoclast differentiation
• The TRANCE-RANK interaction regulates the expression of the anti-apoptotic molecule Bcl-xL
• This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation
• This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response
• T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss
• This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis
• Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts
• The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy
• Two alternatively spliced transcript variants have been found.