CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein
• CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth
• CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes
• Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71
• Applications Reported: This OKT9 (OKT-9) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This OKT9 (OKT-9) antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (cat
• 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
• 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes
• Endosomal acidification leads to iron release
• The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor
• Transferrin receptor is necessary for development of erythrocytes and the nervous system
• Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.