CD11b Monoclonal Antibody (M1/70.15), PE-Texas Red, Invitrogen™

Description

• The M1/70.15 monoclonal antibody (mAb) reacts with the alpha-M subunit of Mac-1 (CD11b/CD18)
• For this PE-Texas Red™ conjugate: excitation 488 nm/peak emission 616 (nm)
• Applications for the M1/70.15 mAb include immunoprecipitation, immunostaining for flow cytometry, IHC of acetone-fixed frozen sections, and blocking of CR3-mediated rosetting
• Use dim light during handling, incubation with cells and prior to analysis
• It is recommended that cells be analyzed within 18 hours of staining
• If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted
• Every lot is tested by flow cytometry using freshly harvested mouse blood
• CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18)
• The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3)
• CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain
• CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions
• CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue
• The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages
• Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages
• Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.