CD81 Monoclonal Antibody (Eat-2 (EAT2)), Biotin, eBioscience™, Invitrogen™

Description

• Description: The Eat-2 (EAT2) monoclonal antibody reacts with mouse CD81, a molecule also known as TAPA-1
• CD81 is expressed on CD4^+CD8^+ thymocytes and broadly in the periphery with high level expressed by B cells, NK cells, macrophages and dendritic cells and lower level on resting T cells
• Activation of T cells induces upregulation of CD81
• CD81 is involved in homotypic adhesion of B and T cells as well as costimulation of T cells
• The Eat-2 antibody is reported to cross-react with rat CD81
• Applications Reported: The Eat-2 (EAT2) antibody has been reported for use in flow cytometric analysis
• Applications Tested: The Eat-2 (EAT2) antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filtration: 0.2 μm post-manufacturing filtered
• CD81 (TAPA-1, target of anti-proliferative antibody-1) is a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells
• CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T cells
• In muscles, CD81 promotes cell fusion and myotube maintenance
• CD81 has been also identified as a receptor for the hepatitis C virus
• Like members of the tetraspanin family that include CD9, CD37, CD53, CD63, and CD82, CD81 is a cell-surface proteins that are characterized by the presence of four hydrophobic domains
• The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility
• CD81 is a cell surface glycoprotein that is known to complex with integrins
• CD81 appears to promote muscle cell fusion and support myotube maintenance
• CD81 associates with CD19, CD21, Leu 13, and integrins on cell membrane and acts as a receptor for the envelope protein E2 of chronic hepatitis C virus
• Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas
• CD81 is also localized in the tumor-suppressor gene region and is a candidate gene for malignancies.