CD84 Monoclonal Antibody (2G7), Biotin, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody 2G7 reacts to CD84, a transmembrane protein of the Ig SLAM superfamily
• Expression of CD84 is found on mature B cells, a subset of T cells (mainly CD4+CD45RA+), monocytes and platelets
• CD84 is found on a percentage of human hematopoietic progenitor (CD34+) cells
• Signaling through CD84 via homotypic interactions involves phosphorylation and recruitment of SAP and EAT-2 leading to production of IFNgamma and T cell activation
• Applications Reported: This 2G7 antibody has been reported for use in flow cytometric analysis and immunohistology staining of frozen tissue sections
• Applications Tested: This 2G7 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filtration: 0.2 μm post-manufacturing filtered
• CD84 is expressed on mature B cells and on B cell lines, including pre B cell lines, but not on plasma cell lines
• Immunohistochemical studies demonstrated that CD84 strongly expressed on tissues macrophages
• CD84 is also highly expressed on platelets and, at low levels on peripheral blood T cells
• It is a highly glycosylated protein and belongs to immunoglobulin superfamily
• It may play a role in leukocyte activation.