CD2 Monoclonal Antibody (RM2-5), Biotin, eBioscience™, Invitrogen™

Description

• Description: The RM2-5 monoclonal antibody reacts with the mouse CD2 molecule, an approximately 50-55 kDa cell surface receptor expressed by all mouse lymphocytes
• Expression of the CD2 antigen in the mouse differs from that of the human in that B lineage cells in the mouse from the pre-B cell stage to mature B cells express CD2
• CD2 is a ligand for CD48 in the mouse and is involved in adhesion and activation of T cells
• Applications Reported: The RM2-5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: The RM2-5 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filtration: 0.2 μm post-manufacturing filtered
• CD2 (LFA-2) is a monomeric surface antigen (MW range 45-58 kDa) of the human T-lymphocyte lineage that is expressed on all peripheral blood T cells
• CD2 is one of the earliest T-cell markers, being present on more than 95% of thymocytes and it is also found on some natural killer cells, but not on B lymphocytes
• Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it
• The interaction between CD2 and CD58 stabilizes adhesion between T cells and antigen presenting or target cells
• Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion
• Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58
• Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion
• CD2 deficiency reduces intestinal inflammation and helps to control infection
• Diseases associated with CD2 dysfunction include penis squamous cell carcinoma and immune deficiency due to the absence of the thymus.