CD130 (GP130) Monoclonal Antibody (AN-G30), Biotin, eBioscience™, Invitrogen™

Description

• Description: The AN-G30 monoclonal antibody reacts with human gp130, also known as CD130
• gp130 is the common receptor subunit for the IL-6 family of cytokines, including IL-6, IL-11, IL-27, Oncostatin M, and LIF
• gp130 is expressed on numerous cell types, including T cells, mast cells, and epithelial cells
• Soluble forms of gp130 have been isolated from human serum samples
• Upon ligand binding, gp130 signals via the JAK-STAT, MAPK, and PI3 kinase pathways Applications Reported: This AN-G30 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB)
• Applications Tested: This AN-G30 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Filtration: 0.2 μm post-manufacturing filtered
• The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM)
• This protein functions as a part of the cytokine receptor complex
• The activation of this protein is dependent upon the binding of cytokines to their receptors
• vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein
• Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis
• Alternatively spliced transcript variants encoding distinct isoforms have been described.