IL-21 Monoclonal Antibody (eBio2B2-G20 (2B2-G20)), Biotin, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody eBio2B2-G20 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells
• IL-21 is a 131 amino acid protein most closely related to IL-2 and IL-15
• Expression is found in CD4+ cells upon activation
• In addition to its effect on NK cells, IL-21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen and stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL-15
• Applications Reported: The eBio2B2-G20 antibody has been reported for use in ELISA detection of IL-21 analysis
• Applications Tested: The eBio2B2-G20 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-21 (IL-21) in combination with the purified eBio3A3-N2 (cat 14-7219) antibody for capture and recombinant human IL-21 (cat
• 14-8219) as the standard
• A suitable range of concentrations of this antibody for ELISA capture is 0.5-2 μg/mL
• A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/mL - 60 pg/mL should be included in each ELISA plate
• Filtration: 0.2 μm post-manufacturing filtered
• IL-21 is a 17 kDa immunomodulatory cytokine produced mainly by Natural Killer Cells (NKT), T helper (Th) 17 and T follicular helper (TFH) cells
• In TFH cells, IL-21 expression leads to autocrine signaling through the IL-21 receptor (IL-21R) and STAT3, which leads to additional transcriptional activation by Bcl6
• As with IFN gamma for Th1 and IL-4 for Th2 cells, IL-21 is critical for TFH cell development and effector function
• IL-21 plays a role in T cell-dependent B cell differentiation into plasma cells and memory cells, stimulation of IgG production and induction of apoptotic signaling in naive B cells
• In Th17 cells, IL-21 expression and autocrine feedback through STAT3, IRF4 and ROR gamma t lead to upregulation of the IL-23R, thereby preparing Th17 cells for maturation and maintenance by the inflammatory cytokine IL-23
• While upregulating IRF4 and ROR gamma t, IL-21 also mediates the downregulation of Foxp3
• IL-21 deficient mice are protected from developing colitis upon chemical treatment by their inability to upregulate Th17-associated molecules
• In comparison, elevated levels of IL-21 are present in chemically-induced colitis mouse models.